Journal: Materials Today Bio

Article Title: Muscle homing peptide modified liposomes loaded with EGCG improved skeletal muscle dysfunction by inhibiting inflammation in aging mice

doi: 10.1016/j.mtbio.2025.102265

Figure Lengend Snippet: M12EGLP delays senescence and promotes regeneration of C2C12 cells. (A) Fluorescence images of p53 in various groups. (B) Quantitative analysis of p53 fluorescence intensity across different groups. (C) Fluorescence images of p38 in various groups. (D) Quantitative analysis of p38 fluorescence intensity across different groups. (E–G) Western blot analysis of MyoD, MyoG, and GDF8 protein levels along with their relative expression ratios. All data represented the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 .

Article Snippet: Myostatin (GDF8) and GAPDH antibodies were obtained from Proteintech, while MyoD and MyoG antibodies were sourced from Santa Cruz Biotechnology.

Techniques: Fluorescence, Western Blot, Expressing

Journal: Materials Today Bio

Article Title: Muscle homing peptide modified liposomes loaded with EGCG improved skeletal muscle dysfunction by inhibiting inflammation in aging mice

doi: 10.1016/j.mtbio.2025.102265

Figure Lengend Snippet: M12EGLP improves motor ability and promotes muscle regeneration in aging mice. (A) Schematic representation of D-galactose-induced senescence and subsequent treatments. (B–E) Statistical analysis of hanger test, grid suspension test, slope test, and swimming fatigue test. (F) Weight ratio of gastrocnemius muscle in mice. (G) The proportion of type Ⅰ/Ⅱ fibers fluorescence in gastrocnemius muscle tissue across different groups. (H) Fluorescence images of type Ⅰ and Ⅱ fibers in the gastrocnemius muscle tissue from different experimental groups of mice. (I) Quantitative analysis of muscle fiber cross-sectional area following H&E staining in gastrocnemius muscle tissue from different groups. (J) H&E and Oil Red O staining images of gastrocnemius muscle tissue from various groups of mice. (K) Quantitative analysis of Oil Red O staining in gastrocnemius muscle tissue from different groups. (L–M) Western blot analysis of MyoD, MyoG, and GDF8 protein expression levels and their relative quantification. All data represented the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Myostatin (GDF8) and GAPDH antibodies were obtained from Proteintech, while MyoD and MyoG antibodies were sourced from Santa Cruz Biotechnology.

Techniques: Suspension, Fluorescence, Staining, Western Blot, Expressing, Quantitative Proteomics